AN IN-VITRO STUDY OF ANTIBACTERIAL EFFECTS OF SOURSOP LEAVES EXTRACT (Annona muricata) ON Staphylococcus aureus

Abstract

Soursop (Annona muricata) is claimed to have an antimicrobial effect. This study was conducted to determine the antimicrobial effect of Soursop (Annona muricata) leaves extract and the correlation between its effect based on the inhibition zone measured in diameter and for every increases of the concentration of the extract while using wells diffusion method. A total of 700 gram of leaves was prepared and turned into ethanol extract using maceration technique and then the extract is diluted into various concentration, specifically 20%, 40%, 60%, 80%, and 100%. The process was repeated 3 times in order to obtain 24 samples. Ciprofloxacin was used as a positive control while the aquadest was used as the negative. All samples are incubated for 24 hours. Data obtained was analyzed using Pearson’s r with Confidence interval of 95% and the data was statistically significant (p=0,000). The results shown that the Pearson’s r was positive (variance = 0,731) with every increases of concentration of extract in 1%, the diameter of inhibition zone is also increased by amount of 0,15 mm. The Soursop extract shown its efficacy for every concentration which was tested and increases in concentration are correlated to the increases of the diameter of the inhibition zone.